InterPAD - DETAILS

(+)-berbamine, 16h-1,24:6,9-dietheno-11,15-metheno-2H-pyrido(2',3':17,18)(1,11)dioxacycloeicosino(2,3,4-ij)isoquinolin-12-ol, 3,4,4a,5,16a,17,18,19-octahydro-21,22,26-trimethoxy-4,17-dimethyl-, (4aS,16aR)-, 16h-1,24:6,9-dietheno-11,15-metheno-2H-pyrido(2',3':17,18)(1,11)dioxacycloeicosino(2,3,4-ij)isoquinolin-12-ol, 3,4,4a,5,16a,17,18,19-octahydro-21,22,26-trimethoxy-4,17-dimethyl-, (4aS-(4aR*,16aS*))-, 16H-1,24:6,9-dietheno-11,15-metheno-2H-pyrido(2',3':17,18)(1,11)dioxacycloeicosino(2,3,4-ij)isoquinolin-12-ol, 3,4,4a,5,16a,17,18,19-octahydro-21,22,26-trimethoxy-4,17-dimethyl-, hydrochloride (1:2), (4aS,16aR)-, 16H-1,24:6,9-dietheno-11,15-metheno-2H-pyrido(2',3':17,18)(1,11)dioxacycloeicosino(2,3,4-ij)isoquinolin-12-ol, 3,4,4a,5,16a,17,18,19-octahydro-21,22,26-trimethoxy-4,17-dimethyl-, hydrochloride, hydrate (1:1:4), (4aS,16aR)-, 6,6',7-trimethoxy-2,2'-dimethylberbaman-12-ol, berbaman-12-ol, 6,6',7-trimethoxy-2,2'-dimethyl-, berbaman-12-ol, 6,6',7-trimethoxy-2,2'-dimethyl-, dihydrochloride, berbamine, berbamine dihydrochloride, berbamine hydrochloride, berbamine hydrochloride tetrahydrate, berbamine monohydrochloride, berbamine, (+), berbamine, (1'beta)-isomer, berbamine, (1beta)-isomer, berbamine, monohydrochloride, tetrahydrate, D-berbamine, penduline, pycnamine

No.	Title	Href
1	Blockade of AMPK-Mediated cAMP-PKA-CREB/ATF1 Signaling Synergizes with Aspirin to Inhibit Hepatocellular Carcinoma. Cancers (Basel). 2021 Apr 6;13(7):1738. doi: 10.3390/cancers13071738.	`Click`
2	Berbamine (BBM), a Natural STAT3 Inhibitor, Synergistically Enhances the Antigrowth and Proapoptotic Effects of Sorafenib on Hepatocellular Carcinoma Cells. ACS Omega. 2020 Sep 18;5(38):24838-24847. doi: 10.1021/acsomega.0c03527.	`Click`
3	Targeting Na+ /K+ -ATPase by berbamine and ouabain synergizes with sorafenib to inhibit hepatocellular carcinoma. Br J Pharmacol. 2021 Nov;178(21):4389-4407. doi: 10.1111/bph.15616.	`Click`
4	Synergistic Anticancer Effect of a Combination of Berbamine and Arcyriaflavin A against Glioblastoma Stem-like Cells. Molecules. 2022 Nov 17;27(22):7968. doi: 10.3390/molecules27227968.	`Click`
5	Berberine in combination with cisplatin induces necroptosis and apoptosis in ovarian cancer cells. Biol Res. 2019 Jul 18;52(1):37. doi: 10.1186/s40659-019-0243-6.	`Click`
6	Berbamine Hydrochloride inhibits lysosomal acidification by activating Nox2 to potentiate chemotherapy-induced apoptosis via the ROS-MAPK pathway in human lung carcinoma cells. Cell Biol Toxicol. 2023 Aug;39(4):1297-1317. doi: 10.1007/s10565-022-09756-8.	`Click`

No.

Title

Href

Blockade of AMPK-Mediated cAMP-PKA-CREB/ATF1 Signaling Synergizes with Aspirin to Inhibit Hepatocellular Carcinoma. Cancers (Basel). 2021 Apr 6;13(7):1738. doi: 10.3390/cancers13071738.

Click

Berbamine (BBM), a Natural STAT3 Inhibitor, Synergistically Enhances the Antigrowth and Proapoptotic Effects of Sorafenib on Hepatocellular Carcinoma Cells. ACS Omega. 2020 Sep 18;5(38):24838-24847. doi: 10.1021/acsomega.0c03527.

Click

Targeting Na+ /K+ -ATPase by berbamine and ouabain synergizes with sorafenib to inhibit hepatocellular carcinoma. Br J Pharmacol. 2021 Nov;178(21):4389-4407. doi: 10.1111/bph.15616.

Click

Synergistic Anticancer Effect of a Combination of Berbamine and Arcyriaflavin A against Glioblastoma Stem-like Cells. Molecules. 2022 Nov 17;27(22):7968. doi: 10.3390/molecules27227968.

Click

Berberine in combination with cisplatin induces necroptosis and apoptosis in ovarian cancer cells. Biol Res. 2019 Jul 18;52(1):37. doi: 10.1186/s40659-019-0243-6.

Click

Berbamine Hydrochloride inhibits lysosomal acidification by activating Nox2 to potentiate chemotherapy-induced apoptosis via the ROS-MAPK pathway in human lung carcinoma cells. Cell Biol Toxicol. 2023 Aug;39(4):1297-1317. doi: 10.1007/s10565-022-09756-8.

Click

Name	Berbamine
PubChem CID	275182
Molecular Weight	608.7g/mol
Synonyms	(+)-berbamine, 16h-1,24:6,9-dietheno-11,15-metheno-2H-pyrido(2',3':17,18)(1,11)dioxacycloeicosino(2,3,4-ij)isoquinolin-12-ol, 3,4,4a,5,16a,17,18,19-octahydro-21,22,26-trimethoxy-4,17-dimethyl-, (4aS,16aR)-, 16h-1,24:6,9-dietheno-11,15-metheno-2H-pyrido(2',3':17,18)(1,11)dioxacycloeicosino(2,3,4-ij)isoquinolin-12-ol, 3,4,4a,5,16a,17,18,19-octahydro-21,22,26-trimethoxy-4,17-dimethyl-, (4aS-(4aR,16aS))-, 16H-1,24:6,9-dietheno-11,15-metheno-2H-pyrido(2',3':17,18)(1,11)dioxacycloeicosino(2,3,4-ij)isoquinolin-12-ol, 3,4,4a,5,16a,17,18,19-octahydro-21,22,26-trimethoxy-4,17-dimethyl-, hydrochloride (1:2), (4aS,16aR)-, 16H-1,24:6,9-dietheno-11,15-metheno-2H-pyrido(2',3':17,18)(1,11)dioxacycloeicosino(2,3,4-ij)isoquinolin-12-ol, 3,4,4a,5,16a,17,18,19-octahydro-21,22,26-trimethoxy-4,17-dimethyl-, hydrochloride, hydrate (1:1:4), (4aS,16aR)-, 6,6',7-trimethoxy-2,2'-dimethylberbaman-12-ol, berbaman-12-ol, 6,6',7-trimethoxy-2,2'-dimethyl-, berbaman-12-ol, 6,6',7-trimethoxy-2,2'-dimethyl-, dihydrochloride, berbamine, berbamine dihydrochloride, berbamine hydrochloride, berbamine hydrochloride tetrahydrate, berbamine monohydrochloride, berbamine, (+), berbamine, (1'beta)-isomer, berbamine, (1beta)-isomer, berbamine, monohydrochloride, tetrahydrate, D-berbamine, penduline, pycnamine
Formula	C₃₇H₄₀N₂O₆
SMILES	CN1CCC2=CC(=C3C=C2C1CC4=CC=C(C=C4)OC5=C(C=CC(=C5)CC6C7=C(O3)C(=C(C=C7CCN6C)OC)OC)O)OC
InChI	1S/C37H40N2O6/c1-38-14-12-24-19-32(41-3)33-21-27(24)28(38)16-22-6-9-26(10-7-22)44-31-18-23(8-11-30(31)40)17-29-35-25(13-15-39(29)2)20-34(42-4)36(43-5)37(35)45-33/h6-11,18-21,28-29,40H,12-17H2,1-5H3/t28-,29+/m0/s1
InChIKey	DFOCUWZXJBAUSQ-URLMMPGGSA-N
CAS Number	478-61-5
ChEMBL ID	CHEMBL504323
ChEBI ID	CHEBI:3063
Herb ID	HBIN017888
Drug Bank ID	DB18146
KEGG ID	C09357
Toxicity	Organism	Test Type	Route(Dose)
	rat	LD50	intraperitoneal(165 mg/kg)
	mouse	LD50	intraperitoneal(254 mg/kg)
	rat	LD50	oral(322 mg/kg)
Structure			Download 2D MOL 3D MOL

Chineses Pinyin	BaiYaoZi
Use Part	Tuberoid
Habitat	JiangSu, AnHui, ZheJiang, JiangXi, FuJian, TaiWan, HuNan, GuangDong, GuangXi, GuiZhou
Flavor	Bitter, Pungent
Meridian Tropism	Lung, Stomach

Chineses Pinyin	GuangFangJi
Use Part	Root
Habitat	GuangDong, GuangXi
Flavor	Bitter, Pungent
Meridian Tropism	Bladder, Lung
Species	>Kingdom: Viridiplantae 　-->Phylum: Streptophyta 　　-->Class: Equisetopsida 　　　-->Order: Piperales 　　　　-->Family: Aristolochiaceae 　　　　　-->Genus: Aristolochia 　　　　　　-->Species: Aristolochia fangchi

Chineses Pinyin	HuangLian
Use Part	Rhizome
Habitat	SiChuan, YunNan, HuBei
Flavor	Bitter
Meridian Tropism	Heart, Spleen, Stomach, Liver, Gallbladder, Large intestine
Species	>Kingdom: Viridiplantae 　-->Phylum: Streptophyta 　　-->Class: Equisetopsida 　　　-->Order: Ranunculales 　　　　-->Family: Ranunculaceae 　　　　　-->Genus: Coptis 　　　　　　-->Species: Coptis chinensis

Chineses Pinyin	GongLaoMu
Use Part	Stem
Habitat	Shaanxi, AnHui, HuBei, HuNan, ZheJiang
Flavor	Bitter
Meridian Tropism	Liver, Stomach, Large intestine
Species	>Kingdom: Viridiplantae 　-->Phylum: Streptophyta 　　-->Class: Equisetopsida 　　　-->Order: Ranunculales 　　　　-->Family: Berberidaceae 　　　　　-->Genus: Berberis 　　　　　　-->Species: Berberis bealei

Chineses Pinyin	BanRuiTangSongCao
Use Part	Root, Rhizome
Habitat	HeiLongJiang, JiLin, LiaoNing, BeiJing, TianJin, HeBei, ShanXi, NeiMengGu, Shaanxi, NingXia, GanSu, QingHai, AnHui, HeNan, SiChuan
Flavor	Bitter
Meridian Tropism	Liver, Stomach, Large intestine
Species	>Kingdom: Viridiplantae 　-->Phylum: Streptophyta 　　-->Class: Equisetopsida 　　　-->Order: Ranunculales 　　　　-->Family: Ranunculaceae 　　　　　-->Genus: Thalictrum 　　　　　　-->Species: Thalictrum petaloideum

Chineses Pinyin	DaYeTangSongCao
Use Part	Root, Rhizome
Habitat	ZheJiang, JiangSu, AnHui, JiangXi, HuNan, HeNan
Flavor	Bitter
Meridian Tropism	Large intestine, Liver
Species	>Kingdom: Viridiplantae 　-->Phylum: Streptophyta 　　-->Class: Equisetopsida 　　　-->Order: Ranunculales 　　　　-->Family: Ranunculaceae 　　　　　-->Genus: Thalictrum 　　　　　　-->Species: Thalictrum faberi

Chineses Pinyin	HuaNanGongLaoYe
Use Part	Leaf
Habitat	GanSu, Shaanxi, JiangSu, AnHui, ZheJiang, JiangXi, HeNan, HuBei, HuNan, GuangDong, GuangXi, SiChuan
Flavor	Bitter
Meridian Tropism	Liver, Kidney

Chineses Pinyin	JinSiMaWeiLian
Use Part	Rhizome And Root
Habitat	YunNan
Flavor	Bitter
Meridian Tropism	Heart, Liver, Gallbladder, Large intestine
Species	>Kingdom: Viridiplantae 　-->Phylum: Streptophyta 　　-->Class: Equisetopsida 　　　-->Order: Ranunculales 　　　　-->Family: Ranunculaceae 　　　　　-->Genus: Thalictrum 　　　　　　-->Species: Thalictrum glandulosissimum

Chineses Pinyin	JinXianDiaoWuGui
Use Part	Root Tuber
Habitat	Shaanxi, GanSu, QingHai, NingXia, XinJiang, ZheJiang, JiangSu, TaiWan, SiChuan, GuiZhou, YunNan, XiZang, ChongQing, BeiJing, TianJin, ShanDong, JiangSu, ShangHai, GuangDong, HaiNan, FuJian, Hongkong
Flavor	Bitter

Chineses Pinyin	MaWeiLian
Use Part	Root, Rhizome
Habitat	China
Flavor	Bitter
Meridian Tropism	Heart, Lung, Liver, Gallbladder, Large intestine
Species	>Kingdom: Viridiplantae 　-->Phylum: Streptophyta 　　-->Class: Equisetopsida 　　　-->Order: Ranunculales 　　　　-->Family: Ranunculaceae 　　　　　-->Genus: Thalictrum 　　　　　　-->Species: Thalictrum foliolosum

Chineses Pinyin	RiBenXiaoBo
Use Part	Root, Root Cortex, Stem-Leaf
Habitat	Japan
Species	>Kingdom: Viridiplantae 　-->Phylum: Streptophyta 　　-->Class: Equisetopsida 　　　-->Order: Ranunculales 　　　　-->Family: Berberidaceae 　　　　　-->Genus: Berberis 　　　　　　-->Species: Berberis thunbergii

Chineses Pinyin	ShaoChiXiaoBo
Habitat	Shaanxi, GanSu, SiChuan
Species	>Kingdom: Viridiplantae 　-->Phylum: Streptophyta 　　-->Class: Equisetopsida 　　　-->Order: Ranunculales 　　　　-->Family: Berberidaceae 　　　　　-->Genus: Berberis 　　　　　　-->Species: Berberis potaninii

Chineses Pinyin	TaiWanQianJinTeng
Use Part	Rhizome
Habitat	TaiWan
Flavor	Sweet
Meridian Tropism	Liver, Stomach
Species	>Kingdom: Viridiplantae 　-->Phylum: Streptophyta 　　-->Class: Equisetopsida 　　　-->Order: Ranunculales 　　　　-->Family: Menispermaceae 　　　　　-->Genus: Stephania 　　　　　　-->Species: Stephania sasakii

Chineses Pinyin	XiYeGongLaoYe
Use Part	Leaf
Habitat	HeNan, HuBei, AnHui, JiangSu, ZheJiang, AnHui, SiChuan, Shaanxi
Flavor	Bitter
Meridian Tropism	Liver, Kidney
Species	>Kingdom: Viridiplantae 　-->Phylum: Streptophyta 　　-->Class: Equisetopsida 　　　-->Order: Aquifoliales 　　　　-->Family: Aquifoliaceae 　　　　　-->Genus: Ilex 　　　　　　-->Species: Ilex cornuta

Pair Name	Berbamine, Aspirin
Partner Name	Aspirin
Disease Info	[ICD-11: 2C12]	Hepatocellular carcinoma		Investigative
Gene Regulation	Down-regulation	Phosphorylation	ATF1	hsa466
	Down-regulation	Expression	CCND1	hsa595
	Down-regulation	Expression	CIP2A	hsa57650
	Down-regulation	Phosphorylation	CREB1	hsa1385
In Vitro Model	Hep-G2	Hepatoblastoma	Homo sapiens (Human)	CVCL_0027
In Vitro Model	Hep 3B2.1-7	Childhood hepatocellular carcinoma	Homo sapiens (Human)	CVCL_0326
In Vivo Model	HepG2 cells (4×10⁶ in 125 μL PBS) were subcutaneously injected into 5-week-old BALB/c nude mice.
Result	These data demonstrate that the regulation of cAMP-PKA-CREB/ATF1 signaling represents a noncanonical function of CPS1. Targeting the PKA-CREB/ATF1 axis may be a strategy to improve the therapeutic effects of aspirin on HCC.

Pair Name	Berbamine, Sorafenib
Partner Name	Sorafenib
Disease Info	[ICD-11: 2C12]	Hepatocellular carcinoma		Investigative
Biological Phenomena	Induction-->Apoptosis
Gene Regulation	Down-regulation	Expression	BCL2	hsa596
	Up-regulation	Cleavage	CASP3	hsa836
	Down-regulation	Expression	CCND1	hsa595
	Down-regulation	Expression	CDK4	hsa1019
	Down-regulation	Expression	CDK6	hsa1021
	Up-regulation	Cleavage	PARP1	hsa142
	Down-regulation	Phosphorylation	RB1	hsa5925
	Down-regulation	Phosphorylation	STAT3	hsa6774
In Vitro Model	HCCLM3	Adult hepatocellular carcinoma	Homo sapiens (Human)	CVCL_6832
In Vitro Model	L-02	Papillomavirus-related cervical adenocarcinoma	Homo sapiens (Human)	CVCL_6926
Result	These findings identify a new type of natural STAT3 inhibitor and provide a novel approach to the enhancement of SORA efficacy by blocking the activation of STAT3.

Pair Name	Berbamine, Sorafenib
Partner Name	Sorafenib
Disease Info	[ICD-11: 2C12]	Hepatocellular carcinoma		Investigative
Biological Phenomena	Target-->Na(+)/K(+)-ATPase
Gene Regulation	Up-regulation	Cleavage	CASP3	hsa836
	Down-regulation	Expression	CCND1	hsa595
	Up-regulation	Phosphorylation	EGFR	hsa1956
	Up-regulation	Phosphorylation	IGF1R	hsa3480
	Up-regulation	Phosphorylation	MAPK1	hsa5594
	Up-regulation	Phosphorylation	MAPK14	hsa1432
	Down-regulation	Expression	MCL1	hsa4170
	Down-regulation	Expression	MYC	hsa4609
	Up-regulation	Cleavage	PARP1	hsa142
	Up-regulation	Phosphorylation	SRC	hsa6714
In Vitro Model	Hep-G2	Hepatoblastoma	Homo sapiens (Human)	CVCL_0027
In Vitro Model	SK-HEP-1	Liver and intrahepatic bile duct epithelial neoplasm	Homo sapiens (Human)	CVCL_0525
In Vivo Model	5×10⁶ HepG2 cells in 200 μl PBS were subcutaneously injected into the mice at 6 weeks of age.
Result	Targeting Na+/K+-ATPase by berbamine and ouabain synergizes with sorafenib to inhibit hepatocellular carcinoma

Pair Name	Berbamine, Arcyriaflavin A
Partner Name	Arcyriaflavin A
Disease Info	[ICD-11: 2A00]	Glioblastoma multiforme		Investigative
Biological Phenomena	Induction-->Apoptosis
Gene Regulation	Down-regulation	Phosphorylation	AKT1	hsa207
	Down-regulation	Expression	ALDH1A1	hsa216
	Down-regulation	Phosphorylation	CAMK1G	hsa57172
	Up-regulation	Cleavage	CASP3	hsa836
	Up-regulation	Cleavage	CASP9	hsa842
	Down-regulation	Expression	CCNA2	hsa890
	Down-regulation	Expression	CCNB1	hsa891
	Down-regulation	Expression	CCND1	hsa595
	Down-regulation	Expression	CCNE1	hsa898
	Down-regulation	Expression	CDK1	hsa983
	Down-regulation	Expression	CDK2	hsa1017
	Down-regulation	Expression	CDK4	hsa1019
	Up-regulation	Expression	CDKN1A	hsa1026
	Down-regulation	Expression	ITGA6	hsa3655
	Down-regulation	Phosphorylation	MAPK1	hsa5594
	Down-regulation	Expression	NANOG	hsa79923
	Up-regulation	Cleavage	PARP1	hsa142
	Down-regulation	Expression	POU5F1	hsa5460
	Down-regulation	Expression	PROM1	hsa8842
	Down-regulation	Expression	SOX2	hsa6657
	Down-regulation	Phosphorylation	STAT3	hsa6774
	Up-regulation	Expression	TP53	hsa7157
In Vitro Model	U-87MG ATCC	Glioblastoma	Homo sapiens (Human)	CVCL_0022
In Vitro Model	C6	Rat malignant glioma	Rattus norvegicus (Rat)	CVCL_0194
In Vivo Model	U87MG-derived GSCs (2×10⁶ cells/egg) were mixed with ECM gel (40 μL/egg) in the absence or presence of the compounds (5 μg/egg) and placed onto the CAM (8 eggs per group).
Result	Our findings suggest that a novel combination therapy involving berbamine and ArcA could effectively eradicate glioblastoma stem-like cells.

Chineses Pinyin	XiaXuTangSongCao
Use Part	Rhizome, Root
Habitat	XiZang, YunNan, SiChuan
Flavor	Bitter
Species	>Kingdom: Viridiplantae 　-->Phylum: Streptophyta 　　-->Class: Equisetopsida 　　　-->Order: Ranunculales 　　　　-->Family: Ranunculaceae 　　　　　-->Genus: Thalictrum 　　　　　　-->Species: Thalictrum atriplex

Chineses Pinyin	XianHuangXiaoBo
Use Part	Endoderm Root Bark, Branch Bark
Habitat	Shaanxi, GanSu, QingHai
Species	>Kingdom: Viridiplantae 　-->Phylum: Streptophyta 　　-->Class: Equisetopsida 　　　-->Order: Ranunculales 　　　　-->Family: Berberidaceae 　　　　　-->Genus: Berberis 　　　　　　-->Species: Berberis diaphana

Chineses Pinyin	XiaoGuoTangSongCao
Use Part	Root
Habitat	YunNan
Flavor	Bitter
Meridian Tropism	Lung, Liver, Gallbladder
Species	>Kingdom: Viridiplantae 　-->Phylum: Streptophyta 　　-->Class: Equisetopsida 　　　-->Order: Ranunculales 　　　　-->Family: Ranunculaceae 　　　　　-->Genus: Thalictrum 　　　　　　-->Species: Thalictrum microgynum

Chineses Pinyin	YanGuoCao
Use Part	Root
Habitat	GuiZhou, HuBei, SiChuan, GanSu, Shaanxi, BeiJing, TianJin, HeBei, ShanXi, NeiMengGu, HeiLongJiang, JiLin, LiaoNing
Flavor	Bitter
Meridian Tropism	Lung, Stomach
Species	>Kingdom: Viridiplantae 　-->Phylum: Streptophyta 　　-->Class: Equisetopsida 　　　-->Order: Ranunculales 　　　　-->Family: Ranunculaceae 　　　　　-->Genus: Thalictrum 　　　　　　-->Species: Thalictrum minus

Chineses Pinyin	YingShuiHuangLian
Use Part	Root, Whole Herb
Habitat	JiLin, LiaoNing, HeiLongJiang, ShanXi, GanSu, QingHai, SiChuan, HuBei, NeiMengGu
Flavor	Bitter
Meridian Tropism	Liver, Lung, Large intestine
Species	>Kingdom: Viridiplantae 　-->Phylum: Streptophyta 　　-->Class: Equisetopsida 　　　-->Order: Ranunculales 　　　　-->Family: Ranunculaceae 　　　　　-->Genus: Thalictrum 　　　　　　-->Species: Thalictrum simplex

Chineses Pinyin	ZhiYiXiaoBo
Use Part	Root Cortex, Endothelium
Habitat	GanSu, NingXia, QingHai, NeiMengGu
Species	>Kingdom: Viridiplantae 　-->Phylum: Streptophyta 　　-->Class: Equisetopsida 　　　-->Order: Ranunculales 　　　　-->Family: Berberidaceae 　　　　　-->Genus: Berberis 　　　　　　-->Species: Berberis dubia

Chineses Pinyin	HuangBai
Use Part	Bark
Habitat	HeiLongJiang, JiLin, LiaoNing, BeiJing, TianJin, HeBei, ShanXi, NeiMengGu, ShanDong, JiangSu, ZheJiang
Flavor	Bitter
Meridian Tropism	Bladder; Large Intestine; Kidney
Species	>Kingdom: Viridiplantae 　-->Phylum: Streptophyta 　　-->Class: Equisetopsida 　　　-->Order: Sapindales 　　　　-->Family: Rutaceae 　　　　　-->Genus: Phellodendron 　　　　　　-->Species: Phellodendron amurense

Pair Name	Berbamine, Cisplatin
Partner Name	Cisplatin
Disease Info	[ICD-11: 2B91]	Colorectal cancer		Investigative
Biological Phenomena	Induction-->Necroptosis
Gene Regulation	Up-regulation	Cleavage	CASP3	hsa836
	Up-regulation	Cleavage	CASP8	hsa841
	Up-regulation	Phosphorylation	MLKL	hsa197259
	Up-regulation	Phosphorylation	RIPK3	hsa11035
In Vitro Model	OVCAR-3	High grade ovarian serous adenocarcinoma	Homo sapiens (Human)	CVCL_0465
Result	This study proposed that the combination therapy of BBR and DDP markedly enhanced more ovarian cancer cell death by inducing apoptosis and necroptosis, which may improve the anticancer effect of chemotherapy drugs. The apoptosis involved the caspase-dependent pathway, while the necroptosis involved the activation of the RIPK3-MLKL pathway. We hope our findings might provide a new insight for the potential of BBR as a therapeutic agent in the treatment of ovarian cancer.