Pair Name	Zerumbone, Cisplatin
Partner Name	Cisplatin
Disease Info	[ICD-11: 2C12]	Hepatocellular carcinoma		Investigative
Biological Phenomena	Induction-->Apoptosis
Gene Regulation	Up-regulation	Expression	BAX	hsa581
	Down-regulation	Expression	BCL2	hsa596
	Up-regulation	Expression	CASP3	hsa836
	Up-regulation	Expression	CASP9	hsa842
	Up-regulation	Expression	CDH1	hsa999
	Down-regulation	Expression	JAK2	hsa3717
	Down-regulation	Expression	MMP2	hsa4313
	Up-regulation	Expression	ROS1	hsa6098
	Down-regulation	Expression	STAT3	hsa6774
	Down-regulation	Expression	TWIST1	hsa7291
	Down-regulation	Expression	VEGFA	hsa7422
In Vitro Model	Huh-7	Adult hepatocellular carcinoma	Homo sapiens (Human)	CVCL_0336
Result	The current study indicates that the treatment of 4.62 μM of ZER combined with 1.93 μM of CIS in human liver cancer cells exerts synergistic effects on cell growth inhibition, apoptosis induction, angiogenesis, and invasion by modulating gene expression.

Pair Name	Zerumbone, Celecoxib
Partner Name	Celecoxib
Disease Info	[ICD-11: 2B90]	Colon cancer		Investigative
Biological Phenomena	Induction-->Endoplasmic reticulum stress
Gene Regulation	Up-regulation	Expression	ATF3	hsa467
	Up-regulation	Expression	ATF4	hsa468
	Up-regulation	Cleavage	CASP3	hsa836
	Up-regulation	Expression	DDIT3	hsa1649
	Up-regulation	Phosphorylation	EIF2AK3	hsa9451
	Up-regulation	Phosphorylation	EIF2S1	hsa1965
	Up-regulation	Expression	HSPA5	hsa3309
	Up-regulation	Expression	TNFRSF10B	hsa8795
In Vitro Model	HCT 116	Colon carcinoma	Homo sapiens (Human)	CVCL_0291
	SW480	Colon adenocarcinoma	Homo sapiens (Human)	CVCL_0546
Result	Our results provide novel insights into the role of ATF3 as an essential transcription factor for p53-independent DR5 induction upon both ZER and CCB treatment, and this may be a useful biomarker for TRAIL-based anticancer therapy.

Pair Name	Zerumbone, Paclitaxel
Partner Name	Paclitaxel
Disease Info	[ICD-11: 2C60]	Breast cancer		Investigative
Biological Phenomena	Induction-->ROS-mediated oxidative stress
Gene Regulation	Up-regulation	Expression	BAX	hsa581
	Down-regulation	Expression	BCL2	hsa596
	Up-regulation	Expression	CASP7	hsa840
	Up-regulation	Expression	CASP9	hsa842
In Vitro Model	MCF-7	Invasive breast carcinoma of no special type	Homo sapiens (Human)	CVCL_0031
Result	The prooxidant properties of zerumbone potentially resensitize breast cancer cells to PTX by enhancing intracellular ROS-mediated oxidative stress.

Pair Name	Zerumbone, Gefitinib
Partner Name	Gefitinib
Disease Info	[ICD-11: 2C25]	Lung cancer		Investigative
Biological Phenomena	Induction-->Ferroptosis
Gene Regulation	Down-regulation	Phosphorylation	AKT1	hsa207
	Up-regulation	Expression	BAX	hsa581
	Down-regulation	Expression	BCL2	hsa596
	Down-regulation	Expression	EGFR	hsa1956
	Down-regulation	Expression	GPX4	hsa2879
	Down-regulation	Expression	KDR	hsa3791
	Down-regulation	Expression	SLC7A11	hsa23657
	Down-regulation	Phosphorylation	STAT3	hsa6774
	Up-regulation	Expression	TP53	hsa7157
	Up-regulation	Expression	VEGFA	hsa7422
In Vitro Model	A-549	Lung adenocarcinoma	Homo sapiens (Human)	CVCL_0023
	NCI-H460	Lung large cell carcinoma	Homo sapiens (Human)	CVCL_0459
In Vivo Model	The mice were injected with 1×10⁷ cell/mL A549 cells (0.2ml/mice) into the left axilla.
Result	Our study suggested that zerumbone combined with gefitinib could effectively inhibit lung cancer for multi-model therapies, including the inhibition of tumor growth, angiogenesis, induce cell apoptosis, and ferroptosis.

Pair Name	Zerumbone, Nimesulide
Partner Name	Nimesulide
Disease Info	[ICD-11: 2B90]	Colon cancer		Investigative
Gene Regulation	Down-regulation	Expression	COX2	hsa4513
In Vivo Model	Acute colitis was induced by exposing female ICR mice to 5% DSS in drinking water for 1 week. One week prior to DSS administration, the experimental mice were fed ZER alone, nimesulide (NIM, a selective COX-2 inhibitor) alone, or both in combination (1000 ppm each) for a total of 2 weeks.
Result	Our results suggest that ZER is a novel food factor for mitigating experimental UC and that use of a combination of agents, with different modes of actions, may be an effective anti-inflammatory strategy.

Pair Name	Zerumbone, Cisplatin
Partner Name	Cisplatin
Disease Info	[ICD-11: 2C77]	Cervical cancer		Investigative
In Vitro Model	HeLa	Human papillomavirus-related cervical adenocarcinoma	Homo sapiens (Human)	CVCL_0030
In Vivo Model	Inbred BALB/c mice with CIN were injected peritoneally with isotonic sodium chloride solution (positive control), CIS, ZER, and a combination of both compounds.
Result	The combination of ZER and CIS has modulated the serum level of interleukin 6 when compared with that in mice treated with isotonic sodium chloride solution.