Pair Name	Tectochrysin, Cetuximab
Partner Name	Tectochrysin
Disease Info	[ICD-11: 2B90]	Colon cancer		Investigative
Biological Phenomena	Induction-->Apoptosis
Gene Regulation	Up-regulation	Cleavage	CASP3	hsa836
	Down-regulation	Expression	CD40	hsa958
	Down-regulation	Expression	CXCL8	hsa3576
	Down-regulation	Phosphorylation	EGFR	hsa1956
	Down-regulation	Expression	FOS	hsa2353
	Down-regulation	Phosphorylation	IKBKE	hsa9641
	Down-regulation	Activity	JUN	hsa3725
	Down-regulation	Activity	NFKB1	hsa4790
	Down-regulation	Expression	PTGS2	hsa5743
	Down-regulation	Expression	RELA	hsa5970
In Vitro Model	HCT 116	Colon carcinoma	Homo sapiens (Human)	CVCL_0291
	SW480	Colon adenocarcinoma	Homo sapiens (Human)	CVCL_0546
Result	Our results indicate that combined therapy with lower concentration of cetuximab and tectochrysin could significantly enhance the cancer cell growth inhibitory effect through the inhibition of EGFR signaling.

Pair Name	Platycodin D, Cetuximab
Partner Name	Platycodin D
Disease Info	[ICD-11: 2B91]	Colorectal cancer		Investigative
Biological Phenomena	Inhibition-->Cell metastasis
Gene Regulation	Down-regulation	Expression	CCND1	hsa595
	Down-regulation	Expression	CTNNB1	hsa1499
	Down-regulation	Expression	MMP7	hsa4316
	Down-regulation	Expression	MYC	hsa4609
In Vitro Model	HT-29	Colon adenocarcinoma	Homo sapiens (Human)	CVCL_0320
	Caco-2	Colon adenocarcinoma	Homo sapiens (Human)	CVCL_0025
In Vivo Model	HT29 and CaCo2 cells were trypsinized and injected into the tail vein of nu/nu nude mice with a concentration of 2×10⁵/0.2 ml to establish a model for metastatic lung tumors.
Result	Our findings provide a potential strategy to inhibit CRC metastasis during cetuximab therapy by addition of platycodin D.

Pair Name	Oridonin, Cetuximab
Partner Name	Oridonin
Disease Info	[ICD-11: 2C23.Z]	Laryngeal cancer		Investigative
Biological Phenomena	Induction-->Blockade of cell cycle in G2/M phase
Gene Regulation	Down-regulation	Phosphorylation	EGFR	hsa1956
	Down-regulation	Expression	FAS	hsa355
	Up-regulation	Expression	MAPK8	hsa5599
	Down-regulation	Expression	ROS1	hsa6098
In Vitro Model	HEp-2	Human papillomavirus-related cervical adenocarcinoma	Homo sapiens (Human)	CVCL_1906
	Tu 212	Head and neck squamous cell carcinoma	Homo sapiens (Human)	CVCL_4915
In Vivo Model	Logarithmically growing HEp-2 cells were harvested by trypsinization, and each mouse was given injections of 1×10⁶ cells subcutaneously into the right flank.
Result	Combined oridonin with cetuximab treatment shows synergistic anticancer effects on laryngeal squamous cell carcinoma: Involvement of inhibition of EGFR and activation of reactive oxygen species-mediated JNK pathway

Pair Name	Beta-Elemene, Cetuximab
Partner Name	Beta-Elemene
Disease Info	[ICD-11: 2B91]	Colorectal cancer		Investigative
Biological Phenomena	Induction-->Ferroptosis
Gene Regulation	Up-regulation	Expression	CDH1	hsa999
	Down-regulation	Expression	CDH2	hsa1000
	Down-regulation	Expression	FTH1	hsa2495
	Down-regulation	Expression	GLS	hsa2744
	Down-regulation	Expression	GPX4	hsa2879
	Up-regulation	Expression	HMOX1	hsa3162
	Down-regulation	Expression	MMP9	hsa4318
	Down-regulation	Expression	SLC40A1	KEGG ID N.A.
	Down-regulation	Expression	SLC7A11	hsa23657
	Down-regulation	Expression	SNAI1	hsa6615
	Down-regulation	Expression	SNAI2	hsa6591
	Up-regulation	Expression	TF	KEGG ID N.A.
	Down-regulation	Expression	VIM	hsa7431
In Vitro Model	HCT 116	Colon carcinoma	Homo sapiens (Human)	CVCL_0291
	LoVo	Colon adenocarcinoma	Homo sapiens (Human)	CVCL_0399
In Vivo Model	HCT116-luc cells were digested and washed by cold PBS for three times, and the final concentration was 2.5×10⁶/ml in cold PBS. A volume of 100 μl cell suspension was injected subcutaneously into right dorsal flank of mice.
Result	natural product β-elemene is a new ferroptosis inducer and combinative treatment of β-elemene and cetuximab is sensitive to KRAS mutant CRC cells by inducing ferroptosis and inhibiting EMT, which will hopefully provide a prospective strategy for CRC patients with RAS mutations.

Pair Name	Triptolide, Cetuximab
Partner Name	Triptolide
Disease Info	[ICD-11: 2C25]	Lung cancer		Investigative
Gene Regulation	Down-regulation	Expression	EGFR	hsa1956
In Vitro Model	A-549	Lung adenocarcinoma	Homo sapiens (Human)	CVCL_0023
	NCI-H1299	Lung large cell carcinoma	Homo sapiens (Human)	CVCL_0060
	NCI-H520	Lung squamous cell carcinoma	Homo sapiens (Human)	CVCL_1566
	UM-SCC-6	Tongue squamous cell carcinoma	Homo sapiens (Human)	CVCL_7773
In Vivo Model	To establish xenografts of cancer cell lines, a suspension of 5×10⁶ H1299, UM-SCC6 cancer cells in 0.1 mL RPMI 1640 was injected into the subcutaneous dorsa of mice at the proximal midline.
Result	Cet-TPL represents a potent targeting therapeutic agent against EGFR-overexpressing NSCLC and others.