Pair Name	Toosendanin, Camptothecin
Partner Name	Camptothecin
Disease Info	[ICD-11: 2C77]	Cervical cancer		Investigative
Biological Phenomena	Induction-->Autophagy
Gene Regulation	Down-regulation	Activity	ATP6AP2	hsa10159
	Down-regulation	Expression	CTSB	hsa1508
	Down-regulation	Expression	CTSD	hsa1509
	Up-regulation	Expression	SQSTM1	hsa8878
In Vitro Model	HeLa	Human papillomavirus-related cervical adenocarcinoma	Homo sapiens (Human)	CVCL_0030
	A-549	Lung adenocarcinoma	Homo sapiens (Human)	CVCL_0023
In Vivo Model	1×10⁶ HeLa cells stably expressing mRFP-GFP-LC3 were suspended in 100 μL serum-free culture medium and inoculated into the left axilla.
Result	These results suggest that toosendanin has the potential to be developed into an anti-cancer drug by blocking chemotherapy-induced protective autophagy.

Pair Name	Toosendanin, Irinotecan
Partner Name	Irinotecan
Disease Info	[ICD-11: 2C60]	Breast cancer		Investigative
Biological Phenomena	Induction-->Autophagy
Gene Regulation	Up-regulation	Expression	CASP3	hsa836
	Down-regulation	Expression	CTSB	hsa1508
	Down-regulation	Expression	CTSD	hsa1509
In Vitro Model	MDA-MB-231	Breast adenocarcinoma	Homo sapiens (Human)	CVCL_0062
	MDA-MB-436	Invasive breast carcinoma	Homo sapiens (Human)	CVCL_0623
In Vivo Model	MDA-MB-231 cells stably expressing RFP-GFP-LC3 were suspended in a 1:1 ratio in serum-free DMEM medium with a matrigel basement membrane matrix and inoculated subcutaneously (5×10⁶/site) in the right armpit.
Result	The data showed that TSN blocked 7-ethyl-10-hydroxycamptothecin (SN-38)/irinotecan-induced protective autophagy, and significantly induced apoptosis in TNBC cells and tumor xenograft models when compared to SN-38/irinotecan alone group.

Pair Name	Toosendanin, Regorafenib
Partner Name	Regorafenib
Disease Info	[ICD-11: 2C12]	Hepatocellular carcinoma		Investigative
Biological Phenomena	Inhibition-->Cell metastasis
Gene Regulation	Down-regulation	Expression	DVL2	hsa1856
	Up-regulation	Phosphorylation	GSK3B	hsa2932
	Down-regulation	Expression	MMP2	hsa4313
	Down-regulation	Expression	MMP9	hsa4318
	Down-regulation	Expression	MYC	hsa4609
	Down-regulation	Phosphorylation	STAT3	hsa6774
	Up-regulation	Expression	WWOX	hsa51741
In Vitro Model	MHCC97-L	Adult hepatocellular carcinoma	Homo sapiens (Human)	CVCL_4973
Result	TSN and RGF combination (TRC) synergistically inhibited the proliferation and migration of MHCC-97L cells. The upregulation of WWOX (WW-domain containing oxidoreductase) played a vital role in the HCC cell growth treated with TRC

Pair Name	Toosendanin, Paclitaxel
Partner Name	Paclitaxel
Disease Info	[ICD-11: 2C60]	Breast cancer		Investigative
Biological Phenomena	Induction-->Endoplasmic reticulum stress
Gene Regulation	Down-regulation	Expression	ADORA2A	hsa135
	Down-regulation	Expression	CASP3	hsa836
	Up-regulation	Expression	CDH1	hsa999
	Down-regulation	Expression	CDH2	hsa1000
	Down-regulation	Expression	CTNNB1	hsa1499
	Down-regulation	Expression	PARP1	hsa142
	Down-regulation	Expression	SNAI1	hsa6615
	Down-regulation	Expression	TWIST1	hsa7291
	Down-regulation	Expression	VIM	hsa7431
In Vitro Model	MDA-MB-231	Breast adenocarcinoma	Homo sapiens (Human)	CVCL_0062
	BT-549	Invasive breast carcinoma of no special type	Homo sapiens (Human)	CVCL_1092
	4T1	Malignant neoplasms of the mouse mammary gland	Mus musculus (Mouse)	CVCL_0125
In Vivo Model	2×10⁵ 4T1-fluc-red cells were inoculated in a mammary fat pat of each mouse.
Result	The results suggest that combination of TSN and PTX is superior to PTX alone, suggesting that it may be a promising alternative adjuvant chemotherapy strategy for patients with TNBC, especially those with metastatic TNBC.