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3',5-dihydroxy-3,4',6,7-tetramethoxyflavone, casticin, vitexicarpin, Vx-5 cpd

No.	Title	Href
1	Casticin potentiates TRAIL-induced apoptosis of gastric cancer cells through endoplasmic reticulum stress. PLoS One. 2013;8(3):e58855. doi: 10.1371/journal.pone.0058855.	`Click`
2	Combinational treatment of 5-fluorouracil and casticin induces apoptosis in mouse leukemia WEHI-3 cells in vitro. Environ Toxicol. 2020 Sep;35(9):911-921. doi: 10.1002/tox.22927.	`Click`

No.

Title

Href

Casticin potentiates TRAIL-induced apoptosis of gastric cancer cells through endoplasmic reticulum stress. PLoS One. 2013;8(3):e58855. doi: 10.1371/journal.pone.0058855.

Click

Combinational treatment of 5-fluorouracil and casticin induces apoptosis in mouse leukemia WEHI-3 cells in vitro. Environ Toxicol. 2020 Sep;35(9):911-921. doi: 10.1002/tox.22927.

Click

Name	Casticin
PubChem CID	5315263
Molecular Weight	374.3g/mol
Synonyms	3',5-dihydroxy-3,4',6,7-tetramethoxyflavone, casticin, vitexicarpin, Vx-5 cpd
Formula	C₁₉H₁₈O₈
SMILES	COC1=C(C=C(C=C1)C2=C(C(=O)C3=C(C(=C(C=C3O2)OC)OC)O)OC)O
InChI	1S/C19H18O8/c1-23-11-6-5-9(7-10(11)20)17-19(26-4)16(22)14-12(27-17)8-13(24-2)18(25-3)15(14)21/h5-8,20-21H,1-4H3
InChIKey	PJQLSMYMOKWUJG-UHFFFAOYSA-N
CAS Number	479-91-4
ChEMBL ID	CHEMBL452767
ChEBI ID	CHEBI:69355
Herb ID	HBIN019899
Structure		Download 2D MOL 3D MOL

Chineses Pinyin	ManJingZi
Use Part	Fruit
Habitat	ShanDong, JiangXi, ZheJiang, FuJian, GuangDong, GuangXi
Flavor	Pungent, Bitter
Meridian Tropism	Bladder, Liver, Stomach
Species	>Kingdom: Viridiplantae 　-->Phylum: Streptophyta 　　-->Class: Equisetopsida 　　　-->Order: Lamiales 　　　　-->Family: Lamiaceae 　　　　　-->Genus: Vitex 　　　　　　-->Species: Vitex trifolia

Chineses Pinyin	HuangHuaHao
Use Part	Whole Herb
Habitat	China
Flavor	Bitter
Species	>Kingdom: Viridiplantae 　-->Phylum: Streptophyta 　　-->Class: Equisetopsida 　　　-->Order: Asterales 　　　　-->Family: Asteraceae 　　　　　-->Genus: Artemisia 　　　　　　-->Species: Artemisia annua

Chineses Pinyin	HuangJingYe
Use Part	Leaf
Habitat	HuNan, JiangXi, ZheJiang, FuJian, GuangDong, GuiZhou, YunNan, HaiNan, XiZang, TaiWan
Flavor	Pungent, Bitter
Meridian Tropism	Lung, Stomach, Large intestine
Species	>Kingdom: Viridiplantae 　-->Phylum: Streptophyta 　　-->Class: Equisetopsida 　　　-->Order: Lamiales 　　　　-->Family: Lamiaceae 　　　　　-->Genus: Vitex 　　　　　　-->Species: Vitex negundo

Chineses Pinyin	TongHao
Use Part	Stem-leaf
Species	>Kingdom: Viridiplantae 　-->Phylum: Streptophyta 　　-->Class: Equisetopsida 　　　-->Order: Asterales 　　　　-->Family: Asteraceae 　　　　　-->Genus: Glebionis 　　　　　　-->Species: Glebionis coronaria

Pair Name	Casticin, TNF-related apoptosis inducing ligand
Partner Name	TNF-related apoptosis inducing ligand
Disease Info	[ICD-11: 2B72]	Gastric cancer		Investigative
Biological Phenomena	Induction-->Endoplasmic reticulum stress
Gene Regulation	Down-regulation	Expression	BCL2	hsa596
	Down-regulation	Expression	BCL-xL	hsa598
	Down-regulation	Expression	BIRC5	hsa332
	Down-regulation	Expression	CFLAR	hsa8837
	Up-regulation	Expression	DDIT3	hsa1649
	Up-regulation	Phosphorylation	EIF2S1	hsa1965
	Up-regulation	Expression	ESR1	hsa2099
	Up-regulation	Expression	HSPA5	hsa3309
	Up-regulation	Expression	ROS1	hsa6098
	Up-regulation	Expression	TNFRSF10B	hsa8795
	Down-regulation	Expression	XIAP	hsa331
In Vitro Model	BGC-823	Human papillomavirus-related cervical adenocarcinoma	Homo sapiens (Human)	CVCL_3360
	SGC-7901	Human papillomavirus-related cervical adenocarcinoma	Homo sapiens (Human)	CVCL_0520
	MGC-803	Gastric mucinous adenocarcinoma	Homo sapiens (Human)	CVCL_5334
Result	Casticin enhances TRAIL-induced apoptosis through the downregulation of cell survival proteins and the upregulation of DR5 receptors through actions on the ROS-ER stress-CHOP pathway.

Pair Name	Casticin, Fluorouracil
Partner Name	Fluorouracil
Disease Info	[ICD-11: 2B33.4]	Leukemia		Investigative
Biological Phenomena	Induction-->Mitochondria-mediated apoptosis
Gene Regulation	Up-regulation	Activity	CASP3	hsa836
	Up-regulation	Activity	CASP8	hsa841
	Up-regulation	Activity	CASP9	hsa842
	Up-regulation	Expression	PARP1	hsa142
In Vitro Model	WEHI-3	Mouse leukemia	Mus musculus (Mouse)	CVCL_3622
Result	We may suggest that 5-FU combined with casticin treatment increased apoptotic cell death in WEHI-3 mouse leukemia cells that may undergo mitochondria and caspases signaling pathways in vitro.