InterPAD - DETAILS

.alpha.-hederin, alpha-hederin, Pulsatilla saponin A, Rha-Ara-3beta-hederagenin

No.	Title	Href
1	Chemopreventive effect of α-hederin/carboplatin combination against experimental colon hyperplasia and impact on JNK signaling. Toxicol Mech Methods. 2021 Feb;31(2):138-149. doi: 10.1080/15376516.2020.1849483.	`Click`
2	The Novel Autophagy Inhibitor Alpha-Hederin Promoted Paclitaxel Cytotoxicity by Increasing Reactive Oxygen Species Accumulation in Non-Small Cell Lung Cancer Cells. Int J Mol Sci. 2018 Oct 18;19(10):3221. doi: 10.3390/ijms19103221.	`Click`
3	Combining α-Hederin with cisplatin increases the apoptosis of gastric cancer in vivo and in vitro via mitochondrial related apoptosis pathway. Biomed Pharmacother. 2019 Dec;120:109477. doi: 10.1016/j.biopha.2019.109477.	`Click`

No.

Title

Href

Chemopreventive effect of α-hederin/carboplatin combination against experimental colon hyperplasia and impact on JNK signaling. Toxicol Mech Methods. 2021 Feb;31(2):138-149. doi: 10.1080/15376516.2020.1849483.

Click

The Novel Autophagy Inhibitor Alpha-Hederin Promoted Paclitaxel Cytotoxicity by Increasing Reactive Oxygen Species Accumulation in Non-Small Cell Lung Cancer Cells. Int J Mol Sci. 2018 Oct 18;19(10):3221. doi: 10.3390/ijms19103221.

Click

Combining α-Hederin with cisplatin increases the apoptosis of gastric cancer in vivo and in vitro via mitochondrial related apoptosis pathway. Biomed Pharmacother. 2019 Dec;120:109477. doi: 10.1016/j.biopha.2019.109477.

Click

Name	Alpha-Hederin
PubChem CID	73296
Molecular Weight	751.0g/mol
Synonyms	.alpha.-hederin, alpha-hederin, Pulsatilla saponin A, Rha-Ara-3beta-hederagenin
Formula	C₄₁H₆₆O₁₂
SMILES	CC1C(C(C(C(O1)OC2C(C(COC2OC3CCC4(C(C3(C)CO)CCC5(C4CC=C6C5(CCC7(C6CC(CC7)(C)C)C(=O)O)C)C)C)O)O)O)O)O
InChI	1S/C41H66O12/c1-21-28(44)30(46)31(47)33(51-21)53-32-29(45)24(43)19-50-34(32)52-27-11-12-37(4)25(38(27,5)20-42)10-13-40(7)26(37)9-8-22-23-18-36(2,3)14-16-41(23,35(48)49)17-15-39(22,40)6/h8,21,23-34,42-47H,9-20H2,1-7H3,(H,48,49)/t21-,23-,24-,25+,26+,27-,28-,29-,30+,31+,32+,33-,34-,37-,38-,39+,40+,41-/m0/s1
InChIKey	KEOITPILCOILGM-LLJOFIFVSA-N
CAS Number	27013-91-8
ChEMBL ID	CHEMBL447565
ChEBI ID	CHEBI:69370
KEGG ID	C08954
Toxicity	Organism	Test Type	Route(Dose)
	rat	LD50	intraperitoneal(165 mg/kg)
	mouse	LD50	intraperitoneal(254 mg/kg)
	rat	LD50	oral(322 mg/kg)
Structure			Download 2D MOL 3D MOL

Chineses Pinyin	ChangChunTeng
Use Part	Stem-leaf
Species	>Kingdom: Viridiplantae 　-->Phylum: Streptophyta 　　-->Class: Equisetopsida 　　　-->Order: Apiales 　　　　-->Family: Araliaceae 　　　　　-->Genus: Hedera 　　　　　　-->Species: Hedera nepalensis

Pair Name	Alpha-Hederin, Carboplatin
Partner Name	Carboplatin
Disease Info	[ICD-11: 2B90]	Colon cancer		Investigative
Biological Phenomena	Induction-->Apoptosis
Gene Regulation	Up-regulation	Phosphorylation	AKT1	hsa207
	Up-regulation	Expression	CASP3	hsa836
	Down-regulation	Expression	CCND1	hsa595
	Up-regulation	Phosphorylation	MAPK8	hsa5599
	Up-regulation	Phosphorylation	PIK3CA	hsa5290
In Vivo Model	The running experiment was designed to test the chemopreventive activity of α-hederin when used as an adjuvant to carboplatin in an experimental model of mouse colon hyperplasia induced by 1,2-dimethylhydrazine (DMH). Fifty male Swiss albino mice were classified into five groups: group (I): saline group, group (II): DMH-induced colon hyperplasia control group, group (III): DMH + carboplatin (5 mg/kg) group, group (IV): DMH + α-hederin (80 mg/kg) group, and group (V): DMH + carboplatin (5 mg/kg)+α-hederin (80 mg/kg) group.
Result	Chemopreventive effect of α-hederin/carboplatin combination against experimental colon hyperplasia and impact on JNK signaling

Pair Name	Alpha-Hederin, Paclitaxel
Partner Name	Paclitaxel
Disease Info	[ICD-11: 2C25]	Lung cancer		Investigative
Biological Phenomena	Induction-->Autophagy
Gene Regulation	Up-regulation	Cleavage	CASP3	hsa836
	Up-regulation	Cleavage	PARP1	hsa142
	Up-regulation	Expression	ROS1	hsa6098
	Up-regulation	Expression	SQSTM1	hsa8878
In Vitro Model	NCI-H1299	Lung large cell carcinoma	Homo sapiens (Human)	CVCL_0060
In Vitro Model	NCI-H1650	Minimally invasive lung adenocarcinoma	Homo sapiens (Human)	CVCL_1483
Result	Our findings suggest that α-Hed can increase the killing effect of Tax on NSCLC cells by promoting ROS accumulation, and that combining α-Hed with classical Tax represents a novel strategy for treating NSCLC.

Pair Name	Alpha-Hederin, Cisplatin
Partner Name	Cisplatin
Disease Info	[ICD-11: 2B72]	Gastric cancer		Investigative
Biological Phenomena	Induction-->ROS accumulation
Gene Regulation	Up-regulation	Expression	APAF1	hsa317
	Down-regulation	Expression	BCL2	hsa596
	Down-regulation	Expression	BIRC5	hsa332
	Up-regulation	Expression	CASP3	hsa836
	Up-regulation	Cleavage	CASP3	hsa836
	Up-regulation	Expression	CASP9	hsa842
	Up-regulation	Cleavage	CASP9	hsa842
	Up-regulation	Expression	CYCS	hsa54205
	Down-regulation	Expression	MMP2	hsa4313
	Down-regulation	Expression	MMP9	hsa4318
	Up-regulation	Expression	ROS1	hsa6098
In Vitro Model	SGC-7901	Human papillomavirus-related cervical adenocarcinoma	Homo sapiens (Human)	CVCL_0520
	HGC-27	Gastric carcinoma	Homo sapiens (Human)	CVCL_1279
	MGC-803	Gastric mucinous adenocarcinoma	Homo sapiens (Human)	CVCL_5334
In Vivo Model	The collected HGC-27 cells were washed in serum-free DMEM, suspended in 100 μl of PBS and subcutaneously implanted into the dorsal region of nude mice.
Result	α-Hederin enhances cisplatin-induced anti-tumour effects in GC both in vitro and in vivo by promoting the accumulation of ROS and decreasing MMP. Our data strongly suggested that α-Hederin is a promising candidate for intervention in gastric cancer.