Pair Name	Shikonin, Gefitinib
Disease Info	[ICD-11: 2C25]	Lung cancer		Investigative
Gene Regulation	Down-regulation	Expression	CCND1	hsa595
	Down-regulation	Expression	PKM	hsa5315
	Down-regulation	Phosphorylation	STAT3	hsa6774
In Vitro Model	A-549	Lung adenocarcinoma	Homo sapiens (Human)	CVCL_0023
In Vivo Model	Mice were injected with A549 (1×10⁷) in the dorsal area.
Result	These results provide a promising therapeutic approach for the treatment of wild-type EGFR non-small cell lung cancer.

Pair Name	Shikonin, Gefitinib
Disease Info	[ICD-11: 2C25]	Lung cancer		Investigative
Biological Phenomena	Induction-->ROS generation
Gene Regulation	Down-regulation	Phosphorylation	AKT1	hsa207
	Up-regulation	Expression	BAD	hsa572
	Up-regulation	Expression	BAX	hsa581
	Down-regulation	Expression	BCL2	hsa596
	Down-regulation	Expression	BCL-xL	hsa598
	Up-regulation	Cleavage	CASP3	hsa836
	Down-regulation	Expression	EGFR	hsa1956
	Down-regulation	Phosphorylation	MAPK1	hsa5594
	Up-regulation	Expression	PARP1	hsa142
In Vitro Model	NCI-H1650	Minimally invasive lung adenocarcinoma	Homo sapiens (Human)	CVCL_1483
	NCI-H1975	Lung adenocarcinoma	Homo sapiens (Human)	CVCL_1511
Result	Shikonin-induced cell apoptosis is closely associated with ROS elevation in the cells. These findings indicate that Shikonin can be an effective small molecule treating gefitinib-resistant NSCLC.