Pair Name	Berbamine, Cisplatin
Disease Info	[ICD-11: 2B91]	Colorectal cancer		Investigative
Biological Phenomena	Induction-->Necroptosis
Gene Regulation	Up-regulation	Cleavage	CASP3	hsa836
	Up-regulation	Cleavage	CASP8	hsa841
	Up-regulation	Phosphorylation	MLKL	hsa197259
	Up-regulation	Phosphorylation	RIPK3	hsa11035
In Vitro Model	OVCAR-3	High grade ovarian serous adenocarcinoma	Homo sapiens (Human)	CVCL_0465
Result	This study proposed that the combination therapy of BBR and DDP markedly enhanced more ovarian cancer cell death by inducing apoptosis and necroptosis, which may improve the anticancer effect of chemotherapy drugs. The apoptosis involved the caspase-dependent pathway, while the necroptosis involved the activation of the RIPK3-MLKL pathway. We hope our findings might provide a new insight for the potential of BBR as a therapeutic agent in the treatment of ovarian cancer.

Pair Name	Berbamine, Cisplatin
Disease Info	[ICD-11: 2C25]	Lung cancer		Investigative
Biological Phenomena	Induction-->Apoptosis
Gene Regulation	Up-regulation	Expression	BAX	hsa581
	Down-regulation	Expression	BCL2	hsa596
	Up-regulation	Cleavage	CASP3	hsa836
	Up-regulation	Expression	CYCS	hsa54205
	Up-regulation	Expression	MAP1LC3A	hsa84557
	Up-regulation	Expression	MAP1LC3B	hsa81631
	Up-regulation	Phosphorylation	MAPK14	hsa1432
	Up-regulation	Phosphorylation	MAPK8	hsa5599
	Up-regulation	Cleavage	PARP1	hsa142
	Up-regulation	Expression	SQSTM1	hsa8878
In Vitro Model	NCI-H1299	Lung large cell carcinoma	Homo sapiens (Human)	CVCL_0060
	NCI-H1975	Lung adenocarcinoma	Homo sapiens (Human)	CVCL_1511
In Vivo Model	Cells were injected into the axilla of nude mice at the amount of 5×10⁷ cells/nude mice, and the tumor formation of the transplanted tumor was observed and measured daily.
Result	These findings indicate that Ber might be a promising adjuvant for enhancing the cancer cell killing effect of chemotherapy via the inhibition of autophagy. In this process, Nox2 might be a significant mediator of Ber-induced aberrant lysosomal acidification.