Pair Name	Puerarin, Cisplatin
Partner Name	Cisplatin
Disease Info	[ICD-11: 2C25]	Lung cancer		Investigative
Biological Phenomena	Induction-->Apoptosis
Gene Regulation	Down-regulation	Expression	CCND1	hsa595
	Up-regulation	Expression	CDH1	hsa999
	Down-regulation	Expression	CTNNB1	hsa1499
	Down-regulation	Expression	MKI67	hsa4288
	Down-regulation	Expression	MMP3	hsa4314
	Down-regulation	Expression	MMP9	hsa4318
	Down-regulation	Expression	MYC	hsa4609
	Down-regulation	Expression	PCNA	hsa5111
	Down-regulation	Expression	VIM	hsa7431
	Up-regulation	Activity	WNT3A	hsa89780
In Vitro Model	A-549	Lung adenocarcinoma	Homo sapiens (Human)	CVCL_0023
In Vivo Model	Sixty BALB/c nude mice were subcutaneously injected on the right armpit with A549/DDP cell suspensions (1×10⁷/100 mL) and then raised under the standard conditions.
Result	Taking these results together, we can draw the conclusion that the PUE enhances the anti-tumor effect of DDP on the drug-resistant A549 cancer in vivo and in vitro through activation of the Wnt signaling pathway.

Pair Name	Puerarin, Fluorouracil
Partner Name	Fluorouracil
Disease Info	[ICD-11: 2B70]	Esophageal cancer		Investigative
Biological Phenomena	Induction-->Apoptosis
Gene Regulation	Up-regulation	Expression	CDH1	hsa999
	Down-regulation	Expression	CDH2	hsa1000
In Vitro Model	Eca-109	Esophageal squamous cell carcinoma	Homo sapiens (Human)	CVCL_6898
In Vivo Model	A total of 24 mice were inoculated subcutaneously with injections of 1×10⁷ cells/mouse.
Result	Puerarin combined with 5‑FU exhibited synergistic effects at lower concentrations and promoted apoptosis, but did not increase the side effects of chemotherapy, which indicated that puerarin may be a safe and effective chemosensitive agent in the treatment of human esophageal cancer.

Pair Name	Puerarin, Fluorouracil
Partner Name	Fluorouracil
Disease Info	[ICD-11: 2B72]	Gastric cancer		Investigative
Gene Regulation	Up-regulation	Expression	BAX	hsa581
	Down-regulation	Expression	BCL2	hsa596
In Vitro Model	BGC-823	Human papillomavirus-related cervical adenocarcinoma	Homo sapiens (Human)	CVCL_3360
In Vivo Model	Cells (5×10⁶ cells/mouse) were injected subcutaneously into the right dorsal area.
Result	These findings showed that puerarin and 5‑FU produced a significant synergic effect on gastric cancer cells, while there was no increase in side effects.