Pair Name	Isorhamnetin, Chloroquine
Disease Info	[ICD-11: 2C60]	Breast cancer		Investigative
Biological Phenomena	Induction-->Mitochondrial fission and apoptosis
Gene Regulation	Up-regulation	Phosphorylation	CAMK2G	hsa818
	Up-regulation	Cleavage	CASP3	hsa836
	Up-regulation	Expression	CYCS	hsa54205
	Up-regulation	Phosphorylation	DNM1L	hsa10059
	Up-regulation	Expression	MAP1LC3A	hsa84557
	Up-regulation	Expression	MAP1LC3B	hsa81631
	Up-regulation	Cleavage	PARP1	hsa142
	Down-regulation	Expression	SQSTM1	hsa8878
In Vitro Model	MDA-MB-231	Breast adenocarcinoma	Homo sapiens (Human)	CVCL_0062
	MCF-7	Invasive breast carcinoma of no special type	Homo sapiens (Human)	CVCL_0031
	BT-549	Invasive breast carcinoma of no special type	Homo sapiens (Human)	CVCL_1092
In Vivo Model	MDA-MB-231 cells (4×10⁷) were inoculated in the right legs of mice. After tumor inoculation, the mice were randomly divided into four treatment groups (16 mice per group; six mice were used for body weight and tumor volume measurement, the others for survival analysis).
Result	Our study highlights the critical role of ROS-mediating CaMKII/Drp1 signaling in the regulation of mitochondrial fission and apoptosis induced by combination of CQ/IH. These findings also suggest that IH could potentially be further developed as a novel chemotherapeutic agent. Furthermore, a combination of IH with classic autophagy/mitophagy inhibitor could represent a novel therapeutic strategy for the treatment of TNBC.