Pair Name	Cucurbitacin B, Cisplatin
Disease Info	[ICD-11: 2C94]	Bladder cancer		Investigative
Biological Phenomena	Induction-->Autophagic proteins
Gene Regulation	Down-regulation	Phosphorylation	AKT1	hsa207
	Down-regulation	Phosphorylation	AKT1S1	hsa84335
	Down-regulation	Phosphorylation	BAD	hsa572
	Up-regulation	Expression	BAX	hsa581
	Down-regulation	Expression	BCL2	hsa596
	Up-regulation	Expression	BECN1	hsa8678
	Down-regulation	Phosphorylation	MTOR	hsa2475
	Up-regulation	Expression	PSMD9	hsa5715
	Down-regulation	Phosphorylation	RAF1	hsa5894
In Vitro Model	MB49	Bladder transitional cell carcinoma	Mus musculus (Mouse)	CVCL_7076
In Vivo Model	1×10⁶ MB49 cells in 100 μL cold PBS were injected subcutaneously into the right flank of the each C57BL/6 mouse strain.
Result	Our results showed that CuB may be a new agent that can support conventional treatment in bladder cancer. Our study is important in terms of enlightening new pathways and developing new treatment methods in the treatment of bladder cancer.