Pair Name	Tubeimoside I, Temozolomide
Disease Info	[ICD-11: 2A00]	Glioblastoma multiforme		Investigative
Biological Phenomena	Induction-->Apoptosis
Gene Regulation	Up-regulation	Cleavage	PARP1	hsa142
	Down-regulation	Expression	MGMT	hsa4255
	Down-regulation	Expression	EGFR	hsa1956
	Down-regulation	Phosphorylation	PIK3CA	hsa5290
	Down-regulation	Phosphorylation	RHOBTB2	hsa23221
	Down-regulation	Phosphorylation	AKT1	hsa207
	Down-regulation	Phosphorylation	MTOR	hsa2475
	Down-regulation	Phosphorylation	NFKB1	hsa4790
	Down-regulation	Phosphorylation	RELA	hsa5970
In Vitro Model	U-118MG	Astrocytoma	Homo sapiens (Human)	CVCL_0633
	T98G	Glioblastoma	Homo sapiens (Human)	CVCL_0556
Result	We first demonstrated that synergistic effects of TBMS1 and TMZ induced apoptosis in GBM cells through reducing MGMT expression and inhibiting the EGFR induced PI3K/Akt/mTOR/NF-κB signaling pathway. This study provides a rationale for combined application of TMZ and TBMS1 as a potential chemotherapeutic treatment for MGMT+ GBM patients.