Pair Name	Sulforaphane, Fluorouracil
Disease Info	[ICD-11: 2B90.Z]	Colon cancer		Investigative
Biological Phenomena	Induction-->Apoptosis
Gene Regulation	Up-regulation	Expression	CASP8	hsa841
	Down-regulation	Expression	CASP3	hsa836
In Vitro Model	Caco-2	Colon adenocarcinoma	Homo sapiens (Human)	CVCL_0025
	LNCaP	Prostate carcinoma	Homo sapiens (Human)	CVCL_0395
	HT-29	Colon adenocarcinoma	Homo sapiens (Human)	CVCL_0320
	PC-3	Prostate carcinoma	Homo sapiens (Human)	CVCL_0035
Result	An increased cytostatic effect was observed in case of alyssin while for sulforaphane the synergistic interaction with 5-fluorouracil involved an intensification of apoptotic cell death.

Pair Name	Sulforaphane, Fluorouracil
Disease Info	[ICD-11: 2C60]	Breast cancer		Investigative
Biological Phenomena	Induction-->Autophagy
Gene Regulation	Down-regulation	Expression	TYMS	hsa7298
	Up-regulation	Expression	BAX	hsa581
	Up-regulation	Expression	CASP3	hsa836
	Down-regulation	Expression	CASP8	hsa841
	Down-regulation	Expression	BCL2	hsa596
	Up-regulation	Expression	MAP1LC3B	hsa81631
	Down-regulation	Expression	CCNB1	hsa891
In Vitro Model	MDA-MB-231	Breast adenocarcinoma	Homo sapiens (Human)	CVCL_0062
Result	Studies of the interaction mechanism have revealed that sulforaphane and 5-fluorouracil act synergistically in the MDA-MB-231 cells by inducing autophagic cell death and premature senescence.