Pair Name	Biochanin A, Fluorouracil
Disease Info	[ICD-11: 2C60]	Breast cancer		Investigative
Biological Phenomena	Induction-->Apoptosis
Gene Regulation	Down-regulation	Expression	AKT1	hsa207
	Down-regulation	Expression	BIRC5	hsa332
	Down-regulation	Expression	CCND1	hsa595
	Down-regulation	Expression	ESR1	hsa2099
	Down-regulation	Expression	MMP2	hsa4313
	Down-regulation	Expression	VEGFA	hsa7422
In Vitro Model	MCF-7	Invasive breast carcinoma of no special type	Homo sapiens (Human)	CVCL_0031
	MDA-MB-231	Breast adenocarcinoma	Homo sapiens (Human)	CVCL_0062
In Vivo Model	Ehrlich solid-phase carcinoma was inducedviathe subcutane-ous inoculation of 0.2 ml of ascetic fluid, containing 2.5×10⁶ EACcells, into the right thigh of the hind limb of each mouse.
Result	The synergistic antitumor effect of Bio-A/ 5-FU combination can be, at least partly, attributed to Bio-A-mediated suppression of ER-α/Akt axis and the augmentation of 5-FU-mediated proapoptotic effects.