Pair Name	Thymoquinone, Temozolomide
Disease Info	[ICD-11: 2A00]	Glioblastoma multiforme		Investigative
Biological Phenomena	Induction-->Apoptosis
Gene Regulation	Up-regulation	Cleavage	CASP3	hsa836
	Up-regulation	Phosphorylation	MAPK14	hsa1432
	Up-regulation	Expression	MGMT	hsa4255
In Vitro Model	M059J	Glioblastoma	Homo sapiens (Human)	CVCL_0400
	Hs 683	Oligodendroglioma	Homo sapiens (Human)	CVCL_0844
	U-251MG	Astrocytoma	Homo sapiens (Human)	CVCL_0021
In Vivo Model	U251R cells were prepared in a matrix mixture (50% wt/wt Matrigel in DMEM) and xenografted to each mouse (1×10⁶ cells in 0.5 ml) under the back skin.
Result	Our findings demonstrate that TQ can effectively cross the BBB and function alone or in combination with TMZ to treat glioblastoma.

Pair Name	Thymoquinone, Temozolomide
Disease Info	[ICD-11: 2A00]	Glioblastoma multiforme		Investigative
Biological Phenomena	Inhibition-->Autophagy
Gene Regulation	Down-regulation	Expression	BECN1	hsa8678
In Vitro Model	U-87MG ATCC	Glioblastoma	Homo sapiens (Human)	CVCL_0022
Result	TQ enhanced the anti-cancer activity of TMZ by inhibition of autophagy at the transcriptional level and decreased the colony-forming ability and NO production of U87MG cell line.