Pair Name	Polyphyllin I, Enzalutamide
Disease Info	[ICD-11: 2C12]	Hepatocellular carcinoma		Investigative
Gene Regulation	Down-regulation	Expression	MUC1	hsa4582
	Down-regulation	Expression	RELA	hsa5970
In Vitro Model	DU145	Prostate carcinoma	Homo sapiens (Human)	CVCL_0105
	PC-3	Prostate carcinoma	Homo sapiens (Human)	CVCL_0035
In Vivo Model	DU145 prostate cancer cells carrying the luciferase report gene (1×10⁶ cells) in 100 µL PBS were implanted via subcutaneous injection into the flanks of the mice.
Result	Our results indicate that PPI inhibits the growth of CRPC cells by inhibiting p65 protein and concomitantly reducing HOTAIR expression, thereby suppressing MUC1 gene expression. The novel regulatory interaction of p65 and HOTAIR converge in the inhibition of MUC1 expression and overall PPI response. The combination of PPI and enzalutamide exhibits synergy. This study reveals a novel mechanism underlying the synergistic inhibitory effect of PPI and enzalutamide on the growth of CRPC cells.